Trypan Blue is indicated for use as an aid in ophthalmic surgery by staining the anterior capsule of the lens.

Trypan Blue is a selective tissue staining agent (dye) that stains the anterior capsule of the human crystalline lens. The dye does not penetrate the capsule, allowing clear visualization of the anterior capsule in contrast to the non-stained lens cortex and inner lens material.

The first step to administer Trypan Blue is to inject an air bubble into the anterior chamber using a 26 gauge needle. It prevents dilution of dye by the aqueous humor. Then Trypan Blue is injected into the anterior lens capsule using a tuberculin syringe. Sufficient staining is achieved as soon as the dye has contracted the capsule. If some area is left unstained, inject one more drop till it gets stained.

Trypan Blue is contraindicated when a non-hydrated (dry state), hydrophilic acrylic intraocular lens (IOL) is planned to be inserted into the eye, as the dye may be absorbed by the IOL and stain it.

• Discoloration of high water content hydrophilic intraocular lenses.
• Inadvertent staining of the posterior lens capsule and vitreous face.
• These effects are generally self-limited, lasting up to one week.

Pregnancy Category C. There are no adequate and well-controlled studies in pregnant women. Trypan Blue should be used in pregnancy only if the potential benefit justifies the potential risk to the fetus. It is not known whether this drug is excreted in human milk. Caution should be exercised when administered to nursing women.

After injection, all excess Trypan Blue should be immediately removed from the eye by thorough irrigation of the anterior chamber.

Pediatric use: The safety and effectiveness of Trypan Blue have been established in pediatric patients based on adequate and well-controlled studies.

Geriatric use: No overall differences in safety and effectiveness have been observed between elderly and younger patients.

Preparations for ophthalmic diagnosis

Store below 30°C and keep away from light.